BD HorizonTM BV421 Rat Anti-Mouse GM-CSF

Description

Two-color flow cytometric analysis of GM-CSF expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant Mouse IL-2 Cat. No. 550069; 10 ng/ml and Mouse IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse GM-CSF antibody Cat. No. 564747; Right Panel . Two-color flow cytometric dot plots showing correlated expression of GM-CSF or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of GM-CSF expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant Mouse IL-2 Cat. No. 550069; 10 ng/ml and Mouse IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse GM-CSF antibody Cat. No. 564747; Right Panel . Two-color flow cytometric dot plots showing correlated expression of GM-CSF or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of GM-CSF expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant Mouse IL-2 Cat. No. 550069; 10 ng/ml and Mouse IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse GM-CSF antibody Cat. No. 564747; Right Panel . Two-color flow cytometric dot plots showing correlated expression of GM-CSF or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of GM-CSF expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant Mouse IL-2 Cat. No. 550069; 10 ng/ml and Mouse IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse GM-CSF antibody Cat. No. 564747; Right Panel . Two-color flow cytometric dot plots showing correlated expression of GM-CSF or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of GM-CSF expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant Mouse IL-2 Cat. No. 550069; 10 ng/ml and Mouse IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse GM-CSF antibody Cat. No. 564747; Right Panel . Two-color flow cytometric dot plots showing correlated expression of GM-CSF or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of GM-CSF expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant Mouse IL-2 Cat. No. 550069; 10 ng/ml and Mouse IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse GM-CSF antibody Cat. No. 564747; Right Panel . Two-color flow cytometric dot plots showing correlated expression of GM-CSF or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.