BD HorizonTM BV421 Rat Anti-Mouse Granzyme C

Description

Multicolor flow cytometric analysis of Granzyme C expression in activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were activated by culture with Recombinant Mouse IL-15 protein 100 ng/ml for 4 days 37C . The cells were harvested, washed, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and BD Horizon Fixable Viability Stain 780 Cat. No. 565388 . The cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse Granzyme C antibody Cat. No. 569861/569937; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Granzyme C or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable Fixable Viability Stain 780-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Granzyme C expression in activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were activated by culture with Recombinant Mouse IL-15 protein 100 ng/ml for 4 days 37C . The cells were harvested, washed, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and BD Horizon Fixable Viability Stain 780 Cat. No. 565388 . The cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse Granzyme C antibody Cat. No. 569861/569937; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Granzyme C or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable Fixable Viability Stain 780-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Granzyme C expression in activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were activated by culture with Recombinant Mouse IL-15 protein 100 ng/ml for 4 days 37C . The cells were harvested, washed, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and BD Horizon Fixable Viability Stain 780 Cat. No. 565388 . The cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse Granzyme C antibody Cat. No. 569861/569937; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Granzyme C or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable Fixable Viability Stain 780-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Granzyme C expression in activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were activated by culture with Recombinant Mouse IL-15 protein 100 ng/ml for 4 days 37C . The cells were harvested, washed, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and BD Horizon Fixable Viability Stain 780 Cat. No. 565388 . The cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse Granzyme C antibody Cat. No. 569861/569937; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Granzyme C or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable Fixable Viability Stain 780-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Granzyme C expression in activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were activated by culture with Recombinant Mouse IL-15 protein 100 ng/ml for 4 days 37C . The cells were harvested, washed, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and BD Horizon Fixable Viability Stain 780 Cat. No. 565388 . The cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse Granzyme C antibody Cat. No. 569861/569937; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Granzyme C or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable Fixable Viability Stain 780-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Granzyme C expression in activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were activated by culture with Recombinant Mouse IL-15 protein 100 ng/ml for 4 days 37C . The cells were harvested, washed, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and BD Horizon Fixable Viability Stain 780 Cat. No. 565388 . The cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse Granzyme C antibody Cat. No. 569861/569937; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Granzyme C or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable Fixable Viability Stain 780-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.