BD HorizonTM BV421 Rat Anti-Mouse I-A/I-E

Description

Multicolor flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on splenocytes from positive and negative mouse strains left and center panels . Mouse spleen cells from either M5/114-negative SJL Left Panel or M5/114-positive BALB/c Center Panel mice were stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E Cat. No. 562564 , APC Anti-Mouse CD45R/B220 Cat. No. 553092/561880 and APC Anti-Mouse CD11b Cat. No. 553312/561690 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD45R/B220 and CD11b were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. The M5/114 monoclonal antibody detects I-A d and I-E d MHC class II alloantigens that are expressed on both B cells and macrophages. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of I-A/I-E expression by cells within C57BL/6 mouse spleen right panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562564, pseudo-colored green and Alexa Fluor 647 Rat-anti-Mouse CD19 Cat. No. 557684, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Multicolor flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on splenocytes from positive and negative mouse strains left and center panels . Mouse spleen cells from either M5/114-negative SJL Left Panel or M5/114-positive BALB/c Center Panel mice were stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E Cat. No. 562564 , APC Anti-Mouse CD45R/B220 Cat. No. 553092/561880 and APC Anti-Mouse CD11b Cat. No. 553312/561690 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD45R/B220 and CD11b were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. The M5/114 monoclonal antibody detects I-A d and I-E d MHC class II alloantigens that are expressed on both B cells and macrophages. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of I-A/I-E expression by cells within C57BL/6 mouse spleen right panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562564, pseudo-colored green and Alexa Fluor 647 Rat-anti-Mouse CD19 Cat. No. 557684, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Multicolor flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on splenocytes from positive and negative mouse strains left and center panels . Mouse spleen cells from either M5/114-negative SJL Left Panel or M5/114-positive BALB/c Center Panel mice were stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E Cat. No. 562564 , APC Anti-Mouse CD45R/B220 Cat. No. 553092/561880 and APC Anti-Mouse CD11b Cat. No. 553312/561690 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD45R/B220 and CD11b were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. The M5/114 monoclonal antibody detects I-A d and I-E d MHC class II alloantigens that are expressed on both B cells and macrophages. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of I-A/I-E expression by cells within C57BL/6 mouse spleen right panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562564, pseudo-colored green and Alexa Fluor 647 Rat-anti-Mouse CD19 Cat. No. 557684, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Multicolor flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on splenocytes from positive and negative mouse strains left and center panels . Mouse spleen cells from either M5/114-negative SJL Left Panel or M5/114-positive BALB/c Center Panel mice were stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E Cat. No. 562564 , APC Anti-Mouse CD45R/B220 Cat. No. 553092/561880 and APC Anti-Mouse CD11b Cat. No. 553312/561690 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD45R/B220 and CD11b were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. The M5/114 monoclonal antibody detects I-A d and I-E d MHC class II alloantigens that are expressed on both B cells and macrophages. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of I-A/I-E expression by cells within C57BL/6 mouse spleen right panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562564, pseudo-colored green and Alexa Fluor 647 Rat-anti-Mouse CD19 Cat. No. 557684, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Multicolor flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on splenocytes from positive and negative mouse strains left and center panels . Mouse spleen cells from either M5/114-negative SJL Left Panel or M5/114-positive BALB/c Center Panel mice were stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E Cat. No. 562564 , APC Anti-Mouse CD45R/B220 Cat. No. 553092/561880 and APC Anti-Mouse CD11b Cat. No. 553312/561690 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD45R/B220 and CD11b were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. The M5/114 monoclonal antibody detects I-A d and I-E d MHC class II alloantigens that are expressed on both B cells and macrophages. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of I-A/I-E expression by cells within C57BL/6 mouse spleen right panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562564, pseudo-colored green and Alexa Fluor 647 Rat-anti-Mouse CD19 Cat. No. 557684, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Multicolor flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on splenocytes from positive and negative mouse strains left and center panels . Mouse spleen cells from either M5/114-negative SJL Left Panel or M5/114-positive BALB/c Center Panel mice were stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E Cat. No. 562564 , APC Anti-Mouse CD45R/B220 Cat. No. 553092/561880 and APC Anti-Mouse CD11b Cat. No. 553312/561690 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD45R/B220 and CD11b were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. The M5/114 monoclonal antibody detects I-A d and I-E d MHC class II alloantigens that are expressed on both B cells and macrophages. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of I-A/I-E expression by cells within C57BL/6 mouse spleen right panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562564, pseudo-colored green and Alexa Fluor 647 Rat-anti-Mouse CD19 Cat. No. 557684, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x.