
565808 ·
BDB Bioscience · Cat: 565808
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD3 expression on rat splenic leucocytes. Rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then either not incubated background control staining; dashed line histogram or incubated with Purified Mouse IgG3, kappa Anti-Rat CD3 antibody Cat. No. 554830/559974; solid line histogram . The cells were washed and then stained with BD Horizon BV421 Rat Anti-Mouse IgG3 antibody Cat. No. 565808 . The fluorescence histogram showing CD3 expression or background control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD3 expression on rat splenic leucocytes. Rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then either not incubated background control staining; dashed line histogram or incubated with Purified Mouse IgG3, kappa Anti-Rat CD3 antibody Cat. No. 554830/559974; solid line histogram . The cells were washed and then stained with BD Horizon BV421 Rat Anti-Mouse IgG3 antibody Cat. No. 565808 . The fluorescence histogram showing CD3 expression or background control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD3 expression on rat splenic leucocytes. Rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then either not incubated background control staining; dashed line histogram or incubated with Purified Mouse IgG3, kappa Anti-Rat CD3 antibody Cat. No. 554830/559974; solid line histogram . The cells were washed and then stained with BD Horizon BV421 Rat Anti-Mouse IgG3 antibody Cat. No. 565808 . The fluorescence histogram showing CD3 expression or background control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.