BD HorizonTM BV421 Rat Anti-Mouse IL-17A

Description

Flow cytometric analysis of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated Left and Middle Panels or were not stimulated Right Panel with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. The cells were fixed, permeabilized and subsequently stained with BD Horizon BV421 Rat Anti-Mouse IL-17A Cat. No. 563354; Middle and Right panels or BD Horizon BV421 Rat IgG1 kappa Isotype Control Cat. No. 562868; Left Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color dot plots showing correlated expression patterns of IL-17A or Ig Isotype control staining versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated Left and Middle Panels or were not stimulated Right Panel with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. The cells were fixed, permeabilized and subsequently stained with BD Horizon BV421 Rat Anti-Mouse IL-17A Cat. No. 563354; Middle and Right panels or BD Horizon BV421 Rat IgG1 kappa Isotype Control Cat. No. 562868; Left Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color dot plots showing correlated expression patterns of IL-17A or Ig Isotype control staining versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated Left and Middle Panels or were not stimulated Right Panel with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. The cells were fixed, permeabilized and subsequently stained with BD Horizon BV421 Rat Anti-Mouse IL-17A Cat. No. 563354; Middle and Right panels or BD Horizon BV421 Rat IgG1 kappa Isotype Control Cat. No. 562868; Left Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color dot plots showing correlated expression patterns of IL-17A or Ig Isotype control staining versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated Left and Middle Panels or were not stimulated Right Panel with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. The cells were fixed, permeabilized and subsequently stained with BD Horizon BV421 Rat Anti-Mouse IL-17A Cat. No. 563354; Middle and Right panels or BD Horizon BV421 Rat IgG1 kappa Isotype Control Cat. No. 562868; Left Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color dot plots showing correlated expression patterns of IL-17A or Ig Isotype control staining versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated Left and Middle Panels or were not stimulated Right Panel with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. The cells were fixed, permeabilized and subsequently stained with BD Horizon BV421 Rat Anti-Mouse IL-17A Cat. No. 563354; Middle and Right panels or BD Horizon BV421 Rat IgG1 kappa Isotype Control Cat. No. 562868; Left Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color dot plots showing correlated expression patterns of IL-17A or Ig Isotype control staining versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated Left and Middle Panels or were not stimulated Right Panel with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. The cells were fixed, permeabilized and subsequently stained with BD Horizon BV421 Rat Anti-Mouse IL-17A Cat. No. 563354; Middle and Right panels or BD Horizon BV421 Rat IgG1 kappa Isotype Control Cat. No. 562868; Left Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color dot plots showing correlated expression patterns of IL-17A or Ig Isotype control staining versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.