BD HorizonTM BV421 Rat Anti-Mouse IL-2

Description

Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells Cat No. 554652 were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2b Isotype Control Cat No. 562603, Left Panel or BD Horizon BV421 Rat Anti-Mouse IL-2 Cat No. 562969, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells Cat No. 554652 were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2b Isotype Control Cat No. 562603, Left Panel or BD Horizon BV421 Rat Anti-Mouse IL-2 Cat No. 562969, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells Cat No. 554652 were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2b Isotype Control Cat No. 562603, Left Panel or BD Horizon BV421 Rat Anti-Mouse IL-2 Cat No. 562969, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells Cat No. 554652 were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2b Isotype Control Cat No. 562603, Left Panel or BD Horizon BV421 Rat Anti-Mouse IL-2 Cat No. 562969, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells Cat No. 554652 were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2b Isotype Control Cat No. 562603, Left Panel or BD Horizon BV421 Rat Anti-Mouse IL-2 Cat No. 562969, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-2 expressed in activated CD4-positive splenocytes. MiCK-1 Mouse Cytokine Positive Control Cells Cat No. 554652 were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and either BD Horizon BV421 Rat IgG2b Isotype Control Cat No. 562603, Left Panel or BD Horizon BV421 Rat Anti-Mouse IL-2 Cat No. 562969, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.