BD HorizonTM BV421 Rat Anti-Mouse IL-33R (ST2)

Description

Two-color flow cytometric analysis of IL-33R ST2 expression by mouse splenocytes. BALB/c mouse splenic leucocytes were stained with BD Horizon Fixable Viability Stain 510 Cat. No. 564406 followed by staining with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse IL-33R ST2 antibody Cat. No. 566309/566310; Right Plot . The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 560401/560402 . Two-color flow cytometric contour plots showing the correlated expression of Foxp3 versus IL-33R ST2 or Ig Isotype control staining were derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-33R ST2 expression by mouse splenocytes. BALB/c mouse splenic leucocytes were stained with BD Horizon Fixable Viability Stain 510 Cat. No. 564406 followed by staining with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse IL-33R ST2 antibody Cat. No. 566309/566310; Right Plot . The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 560401/560402 . Two-color flow cytometric contour plots showing the correlated expression of Foxp3 versus IL-33R ST2 or Ig Isotype control staining were derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-33R ST2 expression by mouse splenocytes. BALB/c mouse splenic leucocytes were stained with BD Horizon Fixable Viability Stain 510 Cat. No. 564406 followed by staining with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse IL-33R ST2 antibody Cat. No. 566309/566310; Right Plot . The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 560401/560402 . Two-color flow cytometric contour plots showing the correlated expression of Foxp3 versus IL-33R ST2 or Ig Isotype control staining were derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IL-33R ST2 expression by mouse splenocytes. BALB/c mouse splenic leucocytes were stained with BD Horizon Fixable Viability Stain 510 Cat. No. 564406 followed by staining with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse IL-33R ST2 antibody Cat. No. 566309/566310; Right Plot . The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 560401/560402 . Two-color flow cytometric contour plots showing the correlated expression of Foxp3 versus IL-33R ST2 or Ig Isotype control staining were derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IL-33R ST2 expression by mouse splenocytes. BALB/c mouse splenic leucocytes were stained with BD Horizon Fixable Viability Stain 510 Cat. No. 564406 followed by staining with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse IL-33R ST2 antibody Cat. No. 566309/566310; Right Plot . The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 560401/560402 . Two-color flow cytometric contour plots showing the correlated expression of Foxp3 versus IL-33R ST2 or Ig Isotype control staining were derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-33R ST2 expression by mouse splenocytes. BALB/c mouse splenic leucocytes were stained with BD Horizon Fixable Viability Stain 510 Cat. No. 564406 followed by staining with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse IL-33R ST2 antibody Cat. No. 566309/566310; Right Plot . The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 560401/560402 . Two-color flow cytometric contour plots showing the correlated expression of Foxp3 versus IL-33R ST2 or Ig Isotype control staining were derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.