BD HorizonTM BV421 Rat Anti-Mouse Ly-6C

Description

Multicolor flow cytometric analysis of Ly-6C expression on mouse splenocytes. Splenocytes from a BALB/c mouse were stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 553031/553030/561966 and with either BD Horizon BV421 Rat IgM, kappa Isotype Control Cat. No. 562708, Left Panel or with BD Horizon BV421 Rat Anti-Mouse Ly-6C antibody Cat. No. 562727, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of Ly-6C or Ig isotype control staining versus CD8a were derived from gated events with the forward and side light-scattering characteristics of viable splenocytes. Flow cytometry was performed with a BD LSR II Flow Cytometry System. Immunohistofluorescent analysis of Ly-6C expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse Ly-6C antibody Cat. No. 562727, pseudo-colored green and Alexa Fluor 488 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 557669, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Multicolor flow cytometric analysis of Ly-6C expression on mouse splenocytes. Splenocytes from a BALB/c mouse were stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 553031/553030/561966 and with either BD Horizon BV421 Rat IgM, kappa Isotype Control Cat. No. 562708, Left Panel or with BD Horizon BV421 Rat Anti-Mouse Ly-6C antibody Cat. No. 562727, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of Ly-6C or Ig isotype control staining versus CD8a were derived from gated events with the forward and side light-scattering characteristics of viable splenocytes. Flow cytometry was performed with a BD LSR II Flow Cytometry System. Show More Immunohistofluorescent analysis of Ly-6C expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse Ly-6C antibody Cat. No. 562727, pseudo-colored green and Alexa Fluor 488 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 557669, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Multicolor flow cytometric analysis of Ly-6C expression on mouse splenocytes. Splenocytes from a BALB/c mouse were stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 553031/553030/561966 and with either BD Horizon BV421 Rat IgM, kappa Isotype Control Cat. No. 562708, Left Panel or with BD Horizon BV421 Rat Anti-Mouse Ly-6C antibody Cat. No. 562727, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of Ly-6C or Ig isotype control staining versus CD8a were derived from gated events with the forward and side light-scattering characteristics of viable splenocytes. Flow cytometry was performed with a BD LSR II Flow Cytometry System. Immunohistofluorescent analysis of Ly-6C expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse Ly-6C antibody Cat. No. 562727, pseudo-colored green and Alexa Fluor 488 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 557669, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x.