BD HorizonTM BV421 Rat Anti-Mouse LY-6G

Description

Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leukocytes Left and Center Panels . BALB/c bone-marrow cells were stained with either BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737; solid line histogram or BD Horizon BV421 rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram . Flow cytometric histograms were derived from gated events based on the light scattering characteristics of viable lymphoid Left Panel or myeloid Center Panel cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Immunohistofluorescent analysis of Ly-6G expression by cells within C57BL/6 mouse spleen Right Panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737, pseudo-colored green , BD Horizon BV480 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 565631, pseudo-colored blue , and Alexa Fluor 647 Rat Anti-Mouse CD4 antibody Cat. No. 557681, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leukocytes Left and Center Panels . BALB/c bone-marrow cells were stained with either BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737; solid line histogram or BD Horizon BV421 rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram . Flow cytometric histograms were derived from gated events based on the light scattering characteristics of viable lymphoid Left Panel or myeloid Center Panel cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Immunohistofluorescent analysis of Ly-6G expression by cells within C57BL/6 mouse spleen Right Panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737, pseudo-colored green , BD Horizon BV480 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 565631, pseudo-colored blue , and Alexa Fluor 647 Rat Anti-Mouse CD4 antibody Cat. No. 557681, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leukocytes Left and Center Panels . BALB/c bone-marrow cells were stained with either BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737; solid line histogram or BD Horizon BV421 rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram . Flow cytometric histograms were derived from gated events based on the light scattering characteristics of viable lymphoid Left Panel or myeloid Center Panel cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Immunohistofluorescent analysis of Ly-6G expression by cells within C57BL/6 mouse spleen Right Panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737, pseudo-colored green , BD Horizon BV480 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 565631, pseudo-colored blue , and Alexa Fluor 647 Rat Anti-Mouse CD4 antibody Cat. No. 557681, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leukocytes Left and Center Panels . BALB/c bone-marrow cells were stained with either BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737; solid line histogram or BD Horizon BV421 rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram . Flow cytometric histograms were derived from gated events based on the light scattering characteristics of viable lymphoid Left Panel or myeloid Center Panel cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Immunohistofluorescent analysis of Ly-6G expression by cells within C57BL/6 mouse spleen Right Panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737, pseudo-colored green , BD Horizon BV480 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 565631, pseudo-colored blue , and Alexa Fluor 647 Rat Anti-Mouse CD4 antibody Cat. No. 557681, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leukocytes Left and Center Panels . BALB/c bone-marrow cells were stained with either BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737; solid line histogram or BD Horizon BV421 rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram . Flow cytometric histograms were derived from gated events based on the light scattering characteristics of viable lymphoid Left Panel or myeloid Center Panel cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Immunohistofluorescent analysis of Ly-6G expression by cells within C57BL/6 mouse spleen Right Panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737, pseudo-colored green , BD Horizon BV480 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 565631, pseudo-colored blue , and Alexa Fluor 647 Rat Anti-Mouse CD4 antibody Cat. No. 557681, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leukocytes Left and Center Panels . BALB/c bone-marrow cells were stained with either BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737; solid line histogram or BD Horizon BV421 rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram . Flow cytometric histograms were derived from gated events based on the light scattering characteristics of viable lymphoid Left Panel or myeloid Center Panel cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Immunohistofluorescent analysis of Ly-6G expression by cells within C57BL/6 mouse spleen Right Panel . A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G antibody Cat. No. 562737, pseudo-colored green , BD Horizon BV480 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 565631, pseudo-colored blue , and Alexa Fluor 647 Rat Anti-Mouse CD4 antibody Cat. No. 557681, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x.