BD HorizonTM BV421 Rat Anti-Mouse Siglec-H

Description

Multicolor flow cytometric analysis of Siglec-H expression on mouse splenocytes and thymocytes. BALB/c mouse splenic leucocytes Top Plots; preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody/Mouse BD Fc Block, Cat. No. 553141/553142 or thymocytes Bottom Plots were stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plots or BD Horizon BV421 Rat Anti-Mouse Siglec-H antibody Cat. No. 567815; Right Plots at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Siglec-H or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Siglec-H expression on mouse splenocytes and thymocytes. BALB/c mouse splenic leucocytes Top Plots; preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody/Mouse BD Fc Block, Cat. No. 553141/553142 or thymocytes Bottom Plots were stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plots or BD Horizon BV421 Rat Anti-Mouse Siglec-H antibody Cat. No. 567815; Right Plots at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Siglec-H or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Siglec-H expression on mouse splenocytes and thymocytes. BALB/c mouse splenic leucocytes Top Plots; preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody/Mouse BD Fc Block, Cat. No. 553141/553142 or thymocytes Bottom Plots were stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plots or BD Horizon BV421 Rat Anti-Mouse Siglec-H antibody Cat. No. 567815; Right Plots at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Siglec-H or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.