BD HorizonTM BV421 Rat Anti-Mouse TCR Vbeta14

Description

Flow cytometric analysis of TCR Vbeta14 expression on viable Mouse splenic T cells. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BV421 Mouse IgM, kappa Isotype Control Cat. No. 562708; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vbeta14 antibody Cat. No. 568788/568789; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate contour plot showing the correlated expression of TCR Vbeta14 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vbeta14 expression on viable Mouse splenic T cells. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BV421 Mouse IgM, kappa Isotype Control Cat. No. 562708; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vbeta14 antibody Cat. No. 568788/568789; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate contour plot showing the correlated expression of TCR Vbeta14 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vbeta14 expression on viable Mouse splenic T cells. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BV421 Mouse IgM, kappa Isotype Control Cat. No. 562708; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vbeta14 antibody Cat. No. 568788/568789; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate contour plot showing the correlated expression of TCR Vbeta14 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vbeta14 expression on viable Mouse splenic T cells. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BV421 Mouse IgM, kappa Isotype Control Cat. No. 562708; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vbeta14 antibody Cat. No. 568788/568789; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate contour plot showing the correlated expression of TCR Vbeta14 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of TCR Vbeta14 expression on viable Mouse splenic T cells. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BV421 Mouse IgM, kappa Isotype Control Cat. No. 562708; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vbeta14 antibody Cat. No. 568788/568789; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate contour plot showing the correlated expression of TCR Vbeta14 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of TCR Vbeta14 expression on viable Mouse splenic T cells. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BV421 Mouse IgM, kappa Isotype Control Cat. No. 562708; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vbeta14 antibody Cat. No. 568788/568789; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate contour plot showing the correlated expression of TCR Vbeta14 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of TCR Vbeta14 expression on viable Mouse splenic T cells. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BV421 Mouse IgM, kappa Isotype Control Cat. No. 562708; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vbeta14 antibody Cat. No. 568788/568789; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate contour plot showing the correlated expression of TCR Vbeta14 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vbeta14 expression on viable Mouse splenic T cells. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BV421 Mouse IgM, kappa Isotype Control Cat. No. 562708; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vbeta14 antibody Cat. No. 568788/568789; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate contour plot showing the correlated expression of TCR Vbeta14 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TCR Vbeta14 expression on viable Mouse splenic T cells. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BV421 Mouse IgM, kappa Isotype Control Cat. No. 562708; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vbeta14 antibody Cat. No. 568788/568789; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate contour plot showing the correlated expression of TCR Vbeta14 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.