BD HorizonTM BV421 Rat Anti-Mouse Treml4

Description

Flow cytometric analysis of Treml4 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a Cat No. 563786 and APC Hamster Anti-Mouse CD11c Cat No. 550261 antibodies, and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; dashed line histogram or BD Horizon BV421 Rat Anti-Mouse TremI4 antibody Cat. No. 569806/569880; solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histogram showing the expression of Treml4 or Ig Isotype control staining was derived from CD8a and CD11c-positive gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Treml4 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a Cat No. 563786 and APC Hamster Anti-Mouse CD11c Cat No. 550261 antibodies, and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; dashed line histogram or BD Horizon BV421 Rat Anti-Mouse TremI4 antibody Cat. No. 569806/569880; solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histogram showing the expression of Treml4 or Ig Isotype control staining was derived from CD8a and CD11c-positive gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Treml4 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a Cat No. 563786 and APC Hamster Anti-Mouse CD11c Cat No. 550261 antibodies, and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; dashed line histogram or BD Horizon BV421 Rat Anti-Mouse TremI4 antibody Cat. No. 569806/569880; solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histogram showing the expression of Treml4 or Ig Isotype control staining was derived from CD8a and CD11c-positive gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.