BD HorizonTM BV421 Rat Anti-Pax-5

Description

Multicolor flow cytometric analysis of Pax-5 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC and BALB/c mouse splenic leucocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon BV421 Rat Anti-Mouse Pax-5 antibody Cat. No. 563190 or BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 . The PBMC were counterstained with APC Mouse Anti-Human CD21 antibody Cat. No. 561767/561357/559867 . The mouse splenic leucocytes were counterstained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Panel A. Human Peripheral Blood Lymphocytes: The two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Left Plot and Pax-5 Right Plot versus CD21 for events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes. Panel B. Mouse Splenocytes: The dot plots show the coexpression patterns of Ig Isotype control staining Left Plot or Pax-5 Right Plot versus CD45R/B220 for events with the light-scatter characteristics of intact mouse splenocytes. Multicolor flow cytometric analysis of Pax-5 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC and BALB/c mouse splenic leucocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon BV421 Rat Anti-Mouse Pax-5 antibody Cat. No. 563190 or BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 . The PBMC were counterstained with APC Mouse Anti-Human CD21 antibody Cat. No. 561767/561357/559867 . The mouse splenic leucocytes were counterstained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Panel A. Human Peripheral Blood Lymphocytes: The two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Left Plot and Pax-5 Right Plot versus CD21 for events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes. Panel B. Mouse Splenocytes: The dot plots show the coexpression patterns of Ig Isotype control staining Left Plot or Pax-5 Right Plot versus CD45R/B220 for events with the light-scatter characteristics of intact mouse splenocytes. Show More Multicolor flow cytometric analysis of Pax-5 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC and BALB/c mouse splenic leucocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon BV421 Rat Anti-Mouse Pax-5 antibody Cat. No. 563190 or BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 . The PBMC were counterstained with APC Mouse Anti-Human CD21 antibody Cat. No. 561767/561357/559867 . The mouse splenic leucocytes were counterstained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Panel A. Human Peripheral Blood Lymphocytes: The two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Left Plot and Pax-5 Right Plot versus CD21 for events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes. Panel B. Mouse Splenocytes: The dot plots show the coexpression patterns of Ig Isotype control staining Left Plot or Pax-5 Right Plot versus CD45R/B220 for events with the light-scatter characteristics of intact mouse splenocytes.