BD HorizonTM BV421 Streptavidin

Description

Flow cytometric analysis of CD45R/B220 expression on mouse lymphocytes. BALB/c mouse splenic leucocytes were stained with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086 followed by BD Horizon BV421 Streptavidin/SAV Cat. No. 563259; solid line histogram or with BD Horizon BV421 Streptavidin alone dashed line histogram . Flow cytometric histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSRII System. Immunohistofluorescent analysis of CD45R/B220 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS. The sections were then treated with an Avidin/Biotin Blocking Kit Vector Laboratories , and stained with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667, pseudo-colored blue , Alexa Fluor 647 Rat Anti-Mouse CD8a antibody Cat. No. 557682, pseudo-colored red , and Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085 . After washing, sections were then stained with BD Horizon BV421 Streptavidin secondary reagent Cat. No. 563259, pseudo-colored green . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Flow cytometric analysis of CD45R/B220 expression on mouse lymphocytes. BALB/c mouse splenic leucocytes were stained with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086 followed by BD Horizon BV421 Streptavidin/SAV Cat. No. 563259; solid line histogram or with BD Horizon BV421 Streptavidin alone dashed line histogram . Flow cytometric histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSRII System. Show More Immunohistofluorescent analysis of CD45R/B220 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS. The sections were then treated with an Avidin/Biotin Blocking Kit Vector Laboratories , and stained with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667, pseudo-colored blue , Alexa Fluor 647 Rat Anti-Mouse CD8a antibody Cat. No. 557682, pseudo-colored red , and Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085 . After washing, sections were then stained with BD Horizon BV421 Streptavidin secondary reagent Cat. No. 563259, pseudo-colored green . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Flow cytometric analysis of CD45R/B220 expression on mouse lymphocytes. BALB/c mouse splenic leucocytes were stained with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086 followed by BD Horizon BV421 Streptavidin/SAV Cat. No. 563259; solid line histogram or with BD Horizon BV421 Streptavidin alone dashed line histogram . Flow cytometric histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSRII System. Immunohistofluorescent analysis of CD45R/B220 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS. The sections were then treated with an Avidin/Biotin Blocking Kit Vector Laboratories , and stained with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667, pseudo-colored blue , Alexa Fluor 647 Rat Anti-Mouse CD8a antibody Cat. No. 557682, pseudo-colored red , and Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085 . After washing, sections were then stained with BD Horizon BV421 Streptavidin secondary reagent Cat. No. 563259, pseudo-colored green . Images were captured on a standard epifluorescence microscope. Original magnification, 20x.