BD HorizonTM BV480 Mouse Anti-Human CD105 (Endoglin)

Description

Multiparameter flow cytometric analysis of CD105 Endoglin expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD105 Endoglin antibody Cat. No. 569803/569877; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD105 Endoglin or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD105 Endoglin expression on U937 cells. Cells from the Human U937 Histiocytic lymphoma, ATCC CRL-1593 cell line were stained with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV480 Mouse Anti-Human CD105 Endoglin antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD105 Endoglin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of 7-AAD-negative viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD105 Endoglin expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD105 Endoglin antibody Cat. No. 569803/569877; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD105 Endoglin or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD105 Endoglin expression on U937 cells. Cells from the Human U937 Histiocytic lymphoma, ATCC CRL-1593 cell line were stained with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV480 Mouse Anti-Human CD105 Endoglin antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD105 Endoglin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of 7-AAD-negative viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD105 Endoglin expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD105 Endoglin antibody Cat. No. 569803/569877; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD105 Endoglin or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD105 Endoglin expression on U937 cells. Cells from the Human U937 Histiocytic lymphoma, ATCC CRL-1593 cell line were stained with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV480 Mouse Anti-Human CD105 Endoglin antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD105 Endoglin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of 7-AAD-negative viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.