
566101 · 100 Tests
BDB Bioscience · Cat: 566101
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multicolor flow cytometric analysis of CD127 expression on human peripheral blood CD4+ T lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 Cat. No. 565994 and PE Mouse Anti-Human CD25 Cat. No. 555432/560989 antibodies, and either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD127 IL-7 Receptor alpha antibody Cat. No. 566101/566158; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD127 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD127 expression on human peripheral blood CD4+ T lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 Cat. No. 565994 and PE Mouse Anti-Human CD25 Cat. No. 555432/560989 antibodies, and either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD127 IL-7 Receptor alpha antibody Cat. No. 566101/566158; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD127 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD127 expression on human peripheral blood CD4+ T lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 Cat. No. 565994 and PE Mouse Anti-Human CD25 Cat. No. 555432/560989 antibodies, and either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD127 IL-7 Receptor alpha antibody Cat. No. 566101/566158; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD127 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD127 expression on human peripheral blood CD4+ T lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 Cat. No. 565994 and PE Mouse Anti-Human CD25 Cat. No. 555432/560989 antibodies, and either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD127 IL-7 Receptor alpha antibody Cat. No. 566101/566158; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD127 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD127 expression on human peripheral blood CD4+ T lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 Cat. No. 565994 and PE Mouse Anti-Human CD25 Cat. No. 555432/560989 antibodies, and either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD127 IL-7 Receptor alpha antibody Cat. No. 566101/566158; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD127 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD127 expression on human peripheral blood CD4+ T lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 Cat. No. 565994 and PE Mouse Anti-Human CD25 Cat. No. 555432/560989 antibodies, and either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD127 IL-7 Receptor alpha antibody Cat. No. 566101/566158; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD127 were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.