BD HorizonTM BV480 Mouse Anti-Human CD25

Description

Two-color flow cytometric analysis of CD25 expression on unstimulated human lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD4 antibody 561841/555349/561840 and either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD25 antibody Cat. No. 566102/566160; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on stimulated human lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV480 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of CD25 expression on unstimulated human lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD4 antibody 561841/555349/561840 and either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD25 antibody Cat. No. 566102/566160; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD25 expression on stimulated human lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV480 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of CD25 expression on unstimulated human lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD4 antibody 561841/555349/561840 and either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Human CD25 antibody Cat. No. 566102/566160; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on stimulated human lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV480 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.