BD HorizonTM BV480 Mouse Anti-Human IgD

Description

Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV480 Mouse IgG2a, kappa Isotype Control Cat. No. 566089; Left Plot or BD Horizon BV480 Mouse Anti-Human IgD antibody Cat. No. 566138/566187; Right Plot . The two-color flow cytometric contour plots showing the correlated expression of IgD or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV480 Mouse IgG2a, kappa Isotype Control Cat. No. 566089; Left Plot or BD Horizon BV480 Mouse Anti-Human IgD antibody Cat. No. 566138/566187; Right Plot . The two-color flow cytometric contour plots showing the correlated expression of IgD or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV480 Mouse IgG2a, kappa Isotype Control Cat. No. 566089; Left Plot or BD Horizon BV480 Mouse Anti-Human IgD antibody Cat. No. 566138/566187; Right Plot . The two-color flow cytometric contour plots showing the correlated expression of IgD or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV480 Mouse IgG2a, kappa Isotype Control Cat. No. 566089; Left Plot or BD Horizon BV480 Mouse Anti-Human IgD antibody Cat. No. 566138/566187; Right Plot . The two-color flow cytometric contour plots showing the correlated expression of IgD or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV480 Mouse IgG2a, kappa Isotype Control Cat. No. 566089; Left Plot or BD Horizon BV480 Mouse Anti-Human IgD antibody Cat. No. 566138/566187; Right Plot . The two-color flow cytometric contour plots showing the correlated expression of IgD or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV480 Mouse IgG2a, kappa Isotype Control Cat. No. 566089; Left Plot or BD Horizon BV480 Mouse Anti-Human IgD antibody Cat. No. 566138/566187; Right Plot . The two-color flow cytometric contour plots showing the correlated expression of IgD or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.