
569831 · 100 Tests
BDB Bioscience · Cat: 569831
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of PD-L2 CD273 expression on viable Human monocyte-derived mature dendritic cells. Human monocyte-derived mature dendritic cells were stained with either BD Horizon BV480 Mouse IgG2a, kappa Isotype Control Cat. No. 566089; dashed line histogram or BD Horizon BV480 Mouse Anti-Human PD-L2 CD273 antibody Cat. No. 569831/569906; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing PD-L2 CD273 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of PD-L2 CD273 expression on viable Human monocyte-derived mature dendritic cells. Human monocyte-derived mature dendritic cells were stained with either BD Horizon BV480 Mouse IgG2a, kappa Isotype Control Cat. No. 566089; dashed line histogram or BD Horizon BV480 Mouse Anti-Human PD-L2 CD273 antibody Cat. No. 569831/569906; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing PD-L2 CD273 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of PD-L2 CD273 expression on viable Human monocyte-derived mature dendritic cells. Human monocyte-derived mature dendritic cells were stained with either BD Horizon BV480 Mouse IgG2a, kappa Isotype Control Cat. No. 566089; dashed line histogram or BD Horizon BV480 Mouse Anti-Human PD-L2 CD273 antibody Cat. No. 569831/569906; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing PD-L2 CD273 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.