BD HorizonTM BV480 Mouse Anti-Mouse TCR Vgamma6

Description

Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , PE Hamster Anti-Mouse gammadelta T-Cell Receptor TCR gammadelta; Cat. No. 553178 , and with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570929/571001; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative CD3e-positive lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , PE Hamster Anti-Mouse gammadelta T-Cell Receptor TCR gammadelta; Cat. No. 553178 , and with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570929/571001; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative CD3e-positive lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , PE Hamster Anti-Mouse gammadelta T-Cell Receptor TCR gammadelta; Cat. No. 553178 , and with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570929/571001; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative CD3e-positive lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , PE Hamster Anti-Mouse gammadelta T-Cell Receptor TCR gammadelta; Cat. No. 553178 , and with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570929/571001; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative CD3e-positive lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , PE Hamster Anti-Mouse gammadelta T-Cell Receptor TCR gammadelta; Cat. No. 553178 , and with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570929/571001; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative CD3e-positive lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 , PE Hamster Anti-Mouse gammadelta T-Cell Receptor TCR gammadelta; Cat. No. 553178 , and with either BD Horizon BV480 Mouse IgG1, kappa Isotype Control Cat. No. 565652; Left Plot or BD Horizon BV480 Mouse Anti-Mouse TCR Vgamma6 antibody Cat. No. 570929/571001; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vgamma6 or Ig Isotype control staining versus TCR gammadelta was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative CD3e-positive lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.