BD HorizonTM BV480 Rat Anti-Mouse CD196 (CCR6)

Description

Multicolor flow cytometric analysis of CD196 CCR6 expression on viable BALB/c Mouse splenic leukocytes. BALB/c Mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either no BD Horizon BV480 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD196 CCR6 antibody Cat. No. 572047/572061; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD196 CCR6 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD196 CCR6 expression on viable BALB/c Mouse splenic leukocytes. BALB/c Mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either no BD Horizon BV480 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD196 CCR6 antibody Cat. No. 572047/572061; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD196 CCR6 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD196 CCR6 expression on viable BALB/c Mouse splenic leukocytes. BALB/c Mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either no BD Horizon BV480 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD196 CCR6 antibody Cat. No. 572047/572061; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD196 CCR6 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD196 CCR6 expression on viable BALB/c Mouse splenic leukocytes. BALB/c Mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either no BD Horizon BV480 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD196 CCR6 antibody Cat. No. 572047/572061; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD196 CCR6 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD196 CCR6 expression on viable BALB/c Mouse splenic leukocytes. BALB/c Mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either no BD Horizon BV480 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD196 CCR6 antibody Cat. No. 572047/572061; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD196 CCR6 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD196 CCR6 expression on viable BALB/c Mouse splenic leukocytes. BALB/c Mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either no BD Horizon BV480 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD196 CCR6 antibody Cat. No. 572047/572061; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD196 CCR6 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.