BD HorizonTM BV480 Rat Anti-Mouse CD25 (IL-2 Receptor alpha)

Description

Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control Cat. No. 565649; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 569544/569545; Middle Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was generated for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control Cat. No. 565649; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 569544/569545; Middle Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was generated for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control Cat. No. 565649; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 569544/569545; Middle Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was generated for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control Cat. No. 565649; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 569544/569545; Middle Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was generated for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control Cat. No. 565649; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 569544/569545; Middle Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was generated for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control Cat. No. 565649; Left Plot or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 569544/569545; Middle Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was generated for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon BV480 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.