BD HorizonTM BV480 Rat Anti-Mouse CD274 (PD-L1)

Description

Multicolor flow cytometric analysis of CD274 PD-L1 expression on resting and activated Mouse splenocytes. BALB/c Mouse splenic leucocytes were either not activated Top Plots or activated Bottom Plots by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057/567115/567114 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553049/553048/561837 and with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control Cat. No. 565649; Left Plots or BD Horizon BV480 Rat Anti-Mouse CD274 PD-L1 antibody Cat. No. 568589/568590; Right Plots at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD274 PD-L1 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD274 PD-L1 expression on resting and activated Mouse splenocytes. BALB/c Mouse splenic leucocytes were either not activated Top Plots or activated Bottom Plots by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057/567115/567114 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553049/553048/561837 and with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control Cat. No. 565649; Left Plots or BD Horizon BV480 Rat Anti-Mouse CD274 PD-L1 antibody Cat. No. 568589/568590; Right Plots at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD274 PD-L1 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD274 PD-L1 expression on resting and activated Mouse splenocytes. BALB/c Mouse splenic leucocytes were either not activated Top Plots or activated Bottom Plots by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057/567115/567114 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553049/553048/561837 and with either BD Horizon BV480 Rat IgG2b, kappa Isotype Control Cat. No. 565649; Left Plots or BD Horizon BV480 Rat Anti-Mouse CD274 PD-L1 antibody Cat. No. 568589/568590; Right Plots at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD274 PD-L1 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.