
565642 ·
BDB Bioscience · Cat: 565642
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Two-color image analysis of CD3 Molecular Complex expression by cells within C57BL/6 mouse spleen. Mouse spleen cryosections 5 mum were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1 PBS, and stained with BD Horizon BV480 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 565642; pseudo-colored green and BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922; pseudo-colored red . Slides were mounted with ProLong Gold and the images were captured on a standard epifluorescence microscope. Original magnification, 20. Two-color image analysis of CD3 Molecular Complex expression by cells within C57BL/6 mouse spleen. Mouse spleen cryosections 5 mum were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1 PBS, and stained with BD Horizon BV480 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 565642; pseudo-colored green and BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922; pseudo-colored red . Slides were mounted with ProLong Gold and the images were captured on a standard epifluorescence microscope. Original magnification, 20. Show More Two-color image analysis of CD3 Molecular Complex expression by cells within C57BL/6 mouse spleen. Mouse spleen cryosections 5 mum were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1 PBS, and stained with BD Horizon BV480 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 565642; pseudo-colored green and BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922; pseudo-colored red . Slides were mounted with ProLong Gold and the images were captured on a standard epifluorescence microscope. Original magnification, 20.