BD HorizonTM BV480 Rat Anti-Mouse I-A/I-E

Description

Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on BALB/c mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Plot or M5/114-positive BALB/c Right Plot mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV480 Rat Anti-Mouse I-A/I-E Cat. No. 566086/566088 and APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 antibodies. Two-color flow cytometric dot plots showing the correlated expression of I-A/I-E MHC class II alloantigens versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on BALB/c mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Plot or M5/114-positive BALB/c Right Plot mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV480 Rat Anti-Mouse I-A/I-E Cat. No. 566086/566088 and APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 antibodies. Two-color flow cytometric dot plots showing the correlated expression of I-A/I-E MHC class II alloantigens versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on BALB/c mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Plot or M5/114-positive BALB/c Right Plot mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV480 Rat Anti-Mouse I-A/I-E Cat. No. 566086/566088 and APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 antibodies. Two-color flow cytometric dot plots showing the correlated expression of I-A/I-E MHC class II alloantigens versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on BALB/c mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Plot or M5/114-positive BALB/c Right Plot mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV480 Rat Anti-Mouse I-A/I-E Cat. No. 566086/566088 and APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 antibodies. Two-color flow cytometric dot plots showing the correlated expression of I-A/I-E MHC class II alloantigens versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on BALB/c mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Plot or M5/114-positive BALB/c Right Plot mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV480 Rat Anti-Mouse I-A/I-E Cat. No. 566086/566088 and APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 antibodies. Two-color flow cytometric dot plots showing the correlated expression of I-A/I-E MHC class II alloantigens versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on BALB/c mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Plot or M5/114-positive BALB/c Right Plot mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV480 Rat Anti-Mouse I-A/I-E Cat. No. 566086/566088 and APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 antibodies. Two-color flow cytometric dot plots showing the correlated expression of I-A/I-E MHC class II alloantigens versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.