BD HorizonTM BV480 Rat Anti-Mouse Ly-6C

Description

Multicolor flow cytometric analysis of Ly-6C expression on Mouse splenic leucocytes. Top Plots: BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 553030/561966 and were either not further stained BD Horizon BV480 Autofluorescence Control; Left Plot or stained with BD Horizon BV480 Rat Anti-Mouse Ly-6C antibody Cat. No. 569438/569439; Right Plot at 0.5 mug/test. Bottom Plots: BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with BD Horizon BUV737 Rat Anti-CD11b antibody Cat. No. 612800 and were either not further stained BD Horizon BV480 Autofluorescence Control; Left Plot or stained with BD Horizon BV480 Rat Anti-Mouse Ly-6C antibody Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a Top Plots or CD11b Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Ly-6C expression on Mouse splenic leucocytes. Top Plots: BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 553030/561966 and were either not further stained BD Horizon BV480 Autofluorescence Control; Left Plot or stained with BD Horizon BV480 Rat Anti-Mouse Ly-6C antibody Cat. No. 569438/569439; Right Plot at 0.5 mug/test. Bottom Plots: BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with BD Horizon BUV737 Rat Anti-CD11b antibody Cat. No. 612800 and were either not further stained BD Horizon BV480 Autofluorescence Control; Left Plot or stained with BD Horizon BV480 Rat Anti-Mouse Ly-6C antibody Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a Top Plots or CD11b Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Ly-6C expression on Mouse splenic leucocytes. Top Plots: BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 553030/561966 and were either not further stained BD Horizon BV480 Autofluorescence Control; Left Plot or stained with BD Horizon BV480 Rat Anti-Mouse Ly-6C antibody Cat. No. 569438/569439; Right Plot at 0.5 mug/test. Bottom Plots: BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with BD Horizon BUV737 Rat Anti-CD11b antibody Cat. No. 612800 and were either not further stained BD Horizon BV480 Autofluorescence Control; Left Plot or stained with BD Horizon BV480 Rat Anti-Mouse Ly-6C antibody Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a Top Plots or CD11b Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.