BD HorizonTM BV480 Streptavidin

Description

Immunofluorescence staining of human U-2 OS cells. Cultured cells from the human U-2 OS Osteosarcoma, ATCC HTB-96 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized 5 min with BD Phosflow Perm Buffer III Cat. No. 558050 , washed with 1x PBS, and blocked with 5 % Goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1x PBS. The cells were then treated using the Avidin/Biotin Blocking Kit Vector Laboratories , and stained with Biotin Anti-PCNA antibody Abcam . After washing, the cells were stained with the second step reagent, BD Horizon BV480 Streptavidin Cat. No. 564876 pseudo-colored green at 2.5 mug/mL. Cells were then stained with Alexa Fluor 488 Mouse Anti-beta-Tubulin antibody Cat No. 558605 pseudo-colored red . DAPI Cat. No. 564907 was used as a nuclear counterstain pseudo-colored blue . Slides were mounted with ProLong Gold and the image was captured on a BD Pathway 435 Cell Analyzer epifluorescence microscope and merged using BD Attovision Software. 20X objective. Immunofluorescence staining of human U-2 OS cells. Cultured cells from the human U-2 OS Osteosarcoma, ATCC HTB-96 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized 5 min with BD Phosflow Perm Buffer III Cat. No. 558050 , washed with 1x PBS, and blocked with 5 % Goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1x PBS. The cells were then treated using the Avidin/Biotin Blocking Kit Vector Laboratories , and stained with Biotin Anti-PCNA antibody Abcam . After washing, the cells were stained with the second step reagent, BD Horizon BV480 Streptavidin Cat. No. 564876 pseudo-colored green at 2.5 mug/mL. Cells were then stained with Alexa Fluor 488 Mouse Anti-beta-Tubulin antibody Cat No. 558605 pseudo-colored red . DAPI Cat. No. 564907 was used as a nuclear counterstain pseudo-colored blue . Slides were mounted with ProLong Gold and the image was captured on a BD Pathway 435 Cell Analyzer epifluorescence microscope and merged using BD Attovision Software. 20X objective. Show More Immunofluorescence staining of human U-2 OS cells. Cultured cells from the human U-2 OS Osteosarcoma, ATCC HTB-96 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized 5 min with BD Phosflow Perm Buffer III Cat. No. 558050 , washed with 1x PBS, and blocked with 5 % Goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1x PBS. The cells were then treated using the Avidin/Biotin Blocking Kit Vector Laboratories , and stained with Biotin Anti-PCNA antibody Abcam . After washing, the cells were stained with the second step reagent, BD Horizon BV480 Streptavidin Cat. No. 564876 pseudo-colored green at 2.5 mug/mL. Cells were then stained with Alexa Fluor 488 Mouse Anti-beta-Tubulin antibody Cat No. 558605 pseudo-colored red . DAPI Cat. No. 564907 was used as a nuclear counterstain pseudo-colored blue . Slides were mounted with ProLong Gold and the image was captured on a BD Pathway 435 Cell Analyzer epifluorescence microscope and merged using BD Attovision Software. 20X objective.