BD HorizonTM BV510 Hamster Anti-Mouse CD69

Description

Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 5 hours at 37C with 10 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich, Cat. No. P-8139 . The cells were then stained with either BD Horizon BV510 Hamster IgG1, lambda1 Isotype Control Cat. No. 562954; dashed line histogram or with the BD Horizon BV510 Hamster anti-Mouse CD69 antibody Cat. No. 563030; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 5 hours at 37C with 10 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich, Cat. No. P-8139 . The cells were then stained with either BD Horizon BV510 Hamster IgG1, lambda1 Isotype Control Cat. No. 562954; dashed line histogram or with the BD Horizon BV510 Hamster anti-Mouse CD69 antibody Cat. No. 563030; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 5 hours at 37C with 10 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich, Cat. No. P-8139 . The cells were then stained with either BD Horizon BV510 Hamster IgG1, lambda1 Isotype Control Cat. No. 562954; dashed line histogram or with the BD Horizon BV510 Hamster anti-Mouse CD69 antibody Cat. No. 563030; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System.