

563221 ·
BDB Bioscience · Cat: 563221
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Two-color flow cytometric analysis of TCRbeta expression on mouse splenocytes. Mouse splenic leucocytes were incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BV510 Hamster IgG2, lambda1 Isotype Control Cat. No. 563085, Left Panel or BD Horizon BV510 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 563221; Right Panel . Two-color flow cytometric dot plots show the correlated expression patterns of TCRbeta or Ig Isotype control staining versus CD4 and CD8a for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of TCRbeta expression on mouse splenocytes. Mouse splenic leucocytes were incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BV510 Hamster IgG2, lambda1 Isotype Control Cat. No. 563085, Left Panel or BD Horizon BV510 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 563221; Right Panel . Two-color flow cytometric dot plots show the correlated expression patterns of TCRbeta or Ig Isotype control staining versus CD4 and CD8a for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of TCRbeta expression on mouse splenocytes. Mouse splenic leucocytes were incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BV510 Hamster IgG2, lambda1 Isotype Control Cat. No. 563085, Left Panel or BD Horizon BV510 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 563221; Right Panel . Two-color flow cytometric dot plots show the correlated expression patterns of TCRbeta or Ig Isotype control staining versus CD4 and CD8a for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of TCRbeta expression on mouse splenocytes. Mouse splenic leucocytes were incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BV510 Hamster IgG2, lambda1 Isotype Control Cat. No. 563085, Left Panel or BD Horizon BV510 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 563221; Right Panel . Two-color flow cytometric dot plots show the correlated expression patterns of TCRbeta or Ig Isotype control staining versus CD4 and CD8a for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of TCRbeta expression on mouse splenocytes. Mouse splenic leucocytes were incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BV510 Hamster IgG2, lambda1 Isotype Control Cat. No. 563085, Left Panel or BD Horizon BV510 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 563221; Right Panel . Two-color flow cytometric dot plots show the correlated expression patterns of TCRbeta or Ig Isotype control staining versus CD4 and CD8a for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of TCRbeta expression on mouse splenocytes. Mouse splenic leucocytes were incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BV510 Hamster IgG2, lambda1 Isotype Control Cat. No. 563085, Left Panel or BD Horizon BV510 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 563221; Right Panel . Two-color flow cytometric dot plots show the correlated expression patterns of TCRbeta or Ig Isotype control staining versus CD4 and CD8a for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.