BD HorizonTM BV510 Mouse Anti-Human CD206 (Mannose Receptor)

Description

Flow cytometric analysis of CD206 Mannose Receptor expression on viable GM-CSF-stimulated monocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with Recombinant Human GM-CSF protein Cat. No. 550068 . The cells were harvested and stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; dashed line histogram or BD Horizon BV510 Mouse Anti-Human CD206 Mannose Receptor antibody Cat. No. 569618; solid line histogram at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD206 Mannose Receptor expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative GM-CSF activated monocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD206 Mannose Receptor expression on viable GM-CSF-stimulated monocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with Recombinant Human GM-CSF protein Cat. No. 550068 . The cells were harvested and stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; dashed line histogram or BD Horizon BV510 Mouse Anti-Human CD206 Mannose Receptor antibody Cat. No. 569618; solid line histogram at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD206 Mannose Receptor expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative GM-CSF activated monocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD206 Mannose Receptor expression on viable GM-CSF-stimulated monocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with Recombinant Human GM-CSF protein Cat. No. 550068 . The cells were harvested and stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; dashed line histogram or BD Horizon BV510 Mouse Anti-Human CD206 Mannose Receptor antibody Cat. No. 569618; solid line histogram at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD206 Mannose Receptor expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative GM-CSF activated monocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.