BD HorizonTM BV510 Mouse Anti-Human CD25

Description

Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Whole blood was stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human CD25 antibody Cat. No. 563352/563351; Middle Panel . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plot shows the correlated expression of CD25 or Ig Isotype control staining versus cellular autofluorescence derived from gated events with the forward light-scatter characteristics of viable lymphocytes. Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV510 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Whole blood was stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human CD25 antibody Cat. No. 563352/563351; Middle Panel . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plot shows the correlated expression of CD25 or Ig Isotype control staining versus cellular autofluorescence derived from gated events with the forward light-scatter characteristics of viable lymphocytes. Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV510 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Whole blood was stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human CD25 antibody Cat. No. 563352/563351; Middle Panel . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plot shows the correlated expression of CD25 or Ig Isotype control staining versus cellular autofluorescence derived from gated events with the forward light-scatter characteristics of viable lymphocytes. Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV510 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Whole blood was stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human CD25 antibody Cat. No. 563352/563351; Middle Panel . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plot shows the correlated expression of CD25 or Ig Isotype control staining versus cellular autofluorescence derived from gated events with the forward light-scatter characteristics of viable lymphocytes. Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV510 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Whole blood was stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human CD25 antibody Cat. No. 563352/563351; Middle Panel . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plot shows the correlated expression of CD25 or Ig Isotype control staining versus cellular autofluorescence derived from gated events with the forward light-scatter characteristics of viable lymphocytes. Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV510 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Whole blood was stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human CD25 antibody Cat. No. 563352/563351; Middle Panel . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plot shows the correlated expression of CD25 or Ig Isotype control staining versus cellular autofluorescence derived from gated events with the forward light-scatter characteristics of viable lymphocytes. Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV510 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.