BD HorizonTM BV510 Mouse Anti-Human CD25 (IL-2 Receptor alpha)

Description

Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Plot or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569013/569014; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Plot or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569013/569014; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Plot or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569013/569014; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Plot or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569013/569014; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Plot or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569013/569014; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Plot or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569013/569014; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV510 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.