
563388 ·
BDB Bioscience · Cat: 563388
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Description
Two-color flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 antibody Cat. No. 557708 and either BD Horizon BV510 Mouse IgG1 Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human Granzyme B antibody Cat. No. 563388 ; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 antibody Cat. No. 557708 and either BD Horizon BV510 Mouse IgG1 Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human Granzyme B antibody Cat. No. 563388 ; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 antibody Cat. No. 557708 and either BD Horizon BV510 Mouse IgG1 Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human Granzyme B antibody Cat. No. 563388 ; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 antibody Cat. No. 557708 and either BD Horizon BV510 Mouse IgG1 Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human Granzyme B antibody Cat. No. 563388 ; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 antibody Cat. No. 557708 and either BD Horizon BV510 Mouse IgG1 Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human Granzyme B antibody Cat. No. 563388 ; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 antibody Cat. No. 557708 and either BD Horizon BV510 Mouse IgG1 Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human Granzyme B antibody Cat. No. 563388 ; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.