BD HorizonTM BV510 Mouse Anti-Human ICOS (CD278)

Description

Flow cytometric analysis of ICOS CD278 expression on unstimulated and stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were either not stimulated Left Plot or stimulated Right Plot by culture with Phytohemagglutinin PHA; Sigma L-1668 for 3 days. The stimulated cells were then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; dashed line histogram or BD Horizon BV510 Mouse Anti-Human ICOS CD278 antibody Cat. No. 569549; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expression of ICOS CD278 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of ICOS CD278 expression on unstimulated and stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were either not stimulated Left Plot or stimulated Right Plot by culture with Phytohemagglutinin PHA; Sigma L-1668 for 3 days. The stimulated cells were then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; dashed line histogram or BD Horizon BV510 Mouse Anti-Human ICOS CD278 antibody Cat. No. 569549; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expression of ICOS CD278 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of ICOS CD278 expression on unstimulated and stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were either not stimulated Left Plot or stimulated Right Plot by culture with Phytohemagglutinin PHA; Sigma L-1668 for 3 days. The stimulated cells were then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; dashed line histogram or BD Horizon BV510 Mouse Anti-Human ICOS CD278 antibody Cat. No. 569549; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expression of ICOS CD278 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of ICOS CD278 expression on unstimulated and stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were either not stimulated Left Plot or stimulated Right Plot by culture with Phytohemagglutinin PHA; Sigma L-1668 for 3 days. The stimulated cells were then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; dashed line histogram or BD Horizon BV510 Mouse Anti-Human ICOS CD278 antibody Cat. No. 569549; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expression of ICOS CD278 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of ICOS CD278 expression on unstimulated and stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were either not stimulated Left Plot or stimulated Right Plot by culture with Phytohemagglutinin PHA; Sigma L-1668 for 3 days. The stimulated cells were then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; dashed line histogram or BD Horizon BV510 Mouse Anti-Human ICOS CD278 antibody Cat. No. 569549; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expression of ICOS CD278 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of ICOS CD278 expression on unstimulated and stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were either not stimulated Left Plot or stimulated Right Plot by culture with Phytohemagglutinin PHA; Sigma L-1668 for 3 days. The stimulated cells were then stained with either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; dashed line histogram or BD Horizon BV510 Mouse Anti-Human ICOS CD278 antibody Cat. No. 569549; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expression of ICOS CD278 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.