BD HorizonTM BV510 Mouse Anti-Human IgG

Description

Two-color flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human IgG antibody Cat. No. 563247; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human IgG antibody Cat. No. 563247; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human IgG antibody Cat. No. 563247; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human IgG antibody Cat. No. 563247; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human IgG antibody Cat. No. 563247; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat. No. 562946; Left Panel or BD Horizon BV510 Mouse Anti-Human IgG antibody Cat. No. 563247; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.