BD HorizonTM BV510 Mouse Anti-Human IL-17A

Description

Two-color flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946, Left Panel or with the BD Horizon BV510 Mouse Anti-Human IL-17A antibody Cat No. 563295, Right Panel in conjunction with an APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946, Left Panel or with the BD Horizon BV510 Mouse Anti-Human IL-17A antibody Cat No. 563295, Right Panel in conjunction with an APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946, Left Panel or with the BD Horizon BV510 Mouse Anti-Human IL-17A antibody Cat No. 563295, Right Panel in conjunction with an APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946, Left Panel or with the BD Horizon BV510 Mouse Anti-Human IL-17A antibody Cat No. 563295, Right Panel in conjunction with an APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946, Left Panel or with the BD Horizon BV510 Mouse Anti-Human IL-17A antibody Cat No. 563295, Right Panel in conjunction with an APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946, Left Panel or with the BD Horizon BV510 Mouse Anti-Human IL-17A antibody Cat No. 563295, Right Panel in conjunction with an APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.