BD HorizonTM BV510 Mouse Anti-Human IL-2

Description

Two-color flow cytometric analysis of IL-2 expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946; Left Panel or with BD Horizon BV510 Mouse Anti-Human IL-2 antibody Cat No. 563265; Right Panel . Two-color flow cytometric dot plots show IL-2 expression or Ig Isotype control staining versus CD3 derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-2 expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946; Left Panel or with BD Horizon BV510 Mouse Anti-Human IL-2 antibody Cat No. 563265; Right Panel . Two-color flow cytometric dot plots show IL-2 expression or Ig Isotype control staining versus CD3 derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-2 expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946; Left Panel or with BD Horizon BV510 Mouse Anti-Human IL-2 antibody Cat No. 563265; Right Panel . Two-color flow cytometric dot plots show IL-2 expression or Ig Isotype control staining versus CD3 derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IL-2 expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946; Left Panel or with BD Horizon BV510 Mouse Anti-Human IL-2 antibody Cat No. 563265; Right Panel . Two-color flow cytometric dot plots show IL-2 expression or Ig Isotype control staining versus CD3 derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IL-2 expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946; Left Panel or with BD Horizon BV510 Mouse Anti-Human IL-2 antibody Cat No. 563265; Right Panel . Two-color flow cytometric dot plots show IL-2 expression or Ig Isotype control staining versus CD3 derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-2 expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and either BD Horizon BV510 Mouse IgG1, kappa Isotype Control Cat No. 562946; Left Panel or with BD Horizon BV510 Mouse Anti-Human IL-2 antibody Cat No. 563265; Right Panel . Two-color flow cytometric dot plots show IL-2 expression or Ig Isotype control staining versus CD3 derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.