BD HorizonTM BV510 Mouse Anti-Human LAG-3 (CD223)

Description

Multicolor flow cytometric analysis of LAG-3 CD223 expression on viable unstimulated Top Plots and stimulated Bottom Plots Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 567109; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Recombinant Human IL-2 Protein Cat. No. 554603; 10 ng/mL . Unstimulated PBMC Top Plots and stimulated PBMC Bottom Plots were stained with PE Mouse Anti-Human CD8 Cat. No. 561950; Left Plots , BD Horizon BV421 Mouse Anti-Human CD279 PD-1 Cat. No. 562516/565935; Right Plots , and BD Horizon BV510 Mouse Anti-Human LAG-3 CD223 Cat. No. 569616 antibodies at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of LAG-3 CD223 versus CD8 Left Plots or LAG-3 CD223 versus CD279 PD-1 Right Plots were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative unstimulated or stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of LAG-3 CD223 expression on viable unstimulated Top Plots and stimulated Bottom Plots Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 567109; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Recombinant Human IL-2 Protein Cat. No. 554603; 10 ng/mL . Unstimulated PBMC Top Plots and stimulated PBMC Bottom Plots were stained with PE Mouse Anti-Human CD8 Cat. No. 561950; Left Plots , BD Horizon BV421 Mouse Anti-Human CD279 PD-1 Cat. No. 562516/565935; Right Plots , and BD Horizon BV510 Mouse Anti-Human LAG-3 CD223 Cat. No. 569616 antibodies at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of LAG-3 CD223 versus CD8 Left Plots or LAG-3 CD223 versus CD279 PD-1 Right Plots were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative unstimulated or stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of LAG-3 CD223 expression on viable unstimulated Top Plots and stimulated Bottom Plots Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 567109; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Recombinant Human IL-2 Protein Cat. No. 554603; 10 ng/mL . Unstimulated PBMC Top Plots and stimulated PBMC Bottom Plots were stained with PE Mouse Anti-Human CD8 Cat. No. 561950; Left Plots , BD Horizon BV421 Mouse Anti-Human CD279 PD-1 Cat. No. 562516/565935; Right Plots , and BD Horizon BV510 Mouse Anti-Human LAG-3 CD223 Cat. No. 569616 antibodies at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of LAG-3 CD223 versus CD8 Left Plots or LAG-3 CD223 versus CD279 PD-1 Right Plots were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative unstimulated or stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.