BD HorizonTM BV510 Mouse Anti-Ki-67

Description

Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and human peripheral blood mononuclear cells PBMC . Proliferating MOLT-4 cells and predominantly noncycling PBMC were fixed and permeabilized separately with 70 % ice cold ethanol, washed, and stained with BD Horizon BV510 Mouse Anti-Ki-67 antibody Cat. No. 563462 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cell Viability Solution Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and human peripheral blood mononuclear cells PBMC . Proliferating MOLT-4 cells and predominantly noncycling PBMC were fixed and permeabilized separately with 70 % ice cold ethanol, washed, and stained with BD Horizon BV510 Mouse Anti-Ki-67 antibody Cat. No. 563462 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cell Viability Solution Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and human peripheral blood mononuclear cells PBMC . Proliferating MOLT-4 cells and predominantly noncycling PBMC were fixed and permeabilized separately with 70 % ice cold ethanol, washed, and stained with BD Horizon BV510 Mouse Anti-Ki-67 antibody Cat. No. 563462 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cell Viability Solution Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and human peripheral blood mononuclear cells PBMC . Proliferating MOLT-4 cells and predominantly noncycling PBMC were fixed and permeabilized separately with 70 % ice cold ethanol, washed, and stained with BD Horizon BV510 Mouse Anti-Ki-67 antibody Cat. No. 563462 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cell Viability Solution Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and human peripheral blood mononuclear cells PBMC . Proliferating MOLT-4 cells and predominantly noncycling PBMC were fixed and permeabilized separately with 70 % ice cold ethanol, washed, and stained with BD Horizon BV510 Mouse Anti-Ki-67 antibody Cat. No. 563462 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cell Viability Solution Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and human peripheral blood mononuclear cells PBMC . Proliferating MOLT-4 cells and predominantly noncycling PBMC were fixed and permeabilized separately with 70 % ice cold ethanol, washed, and stained with BD Horizon BV510 Mouse Anti-Ki-67 antibody Cat. No. 563462 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cell Viability Solution Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.