BD HorizonTM BV510 Rat Anti-Mouse CD39

Description

Multicolor flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. BALB/c Mouse splenocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553729/557307 , APC Rat Anti-Mouse CD25 Cat. No. 557192 antibodies, and with either BD Horizon BV510 Rat IgG2b, kappa Isotype Control Cat. No. 562951; Left Plots or BD Horizon BV510 Rat Anti-Mouse CD39 antibody Cat. No. 570194/570195; Right Plots at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Top Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 or Ig Isotype control staining versus CD25 were derived from CD4-positive viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. BALB/c Mouse splenocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553729/557307 , APC Rat Anti-Mouse CD25 Cat. No. 557192 antibodies, and with either BD Horizon BV510 Rat IgG2b, kappa Isotype Control Cat. No. 562951; Left Plots or BD Horizon BV510 Rat Anti-Mouse CD39 antibody Cat. No. 570194/570195; Right Plots at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Top Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 or Ig Isotype control staining versus CD25 were derived from CD4-positive viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. BALB/c Mouse splenocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553729/557307 , APC Rat Anti-Mouse CD25 Cat. No. 557192 antibodies, and with either BD Horizon BV510 Rat IgG2b, kappa Isotype Control Cat. No. 562951; Left Plots or BD Horizon BV510 Rat Anti-Mouse CD39 antibody Cat. No. 570194/570195; Right Plots at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Top Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 or Ig Isotype control staining versus CD25 were derived from CD4-positive viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.