BD HorizonTM BV510 Rat Anti-Mouse CD86

Description

Flow cytometric analysis of CD86 expression on activated and resting mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated BALB/c splenic leucocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; dashed line histograms or BD Horizon BV510 Rat Anti-Mouse CD86 antibody Cat. No. 563077; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD86 expression on activated and resting mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated BALB/c splenic leucocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; dashed line histograms or BD Horizon BV510 Rat Anti-Mouse CD86 antibody Cat. No. 563077; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD86 expression on activated and resting mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated BALB/c splenic leucocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; dashed line histograms or BD Horizon BV510 Rat Anti-Mouse CD86 antibody Cat. No. 563077; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD86 expression on activated and resting mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated BALB/c splenic leucocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; dashed line histograms or BD Horizon BV510 Rat Anti-Mouse CD86 antibody Cat. No. 563077; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD86 expression on activated and resting mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated BALB/c splenic leucocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; dashed line histograms or BD Horizon BV510 Rat Anti-Mouse CD86 antibody Cat. No. 563077; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD86 expression on activated and resting mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated BALB/c splenic leucocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; dashed line histograms or BD Horizon BV510 Rat Anti-Mouse CD86 antibody Cat. No. 563077; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.