BD HorizonTM BV510 Rat Anti-Mouse CX3CR1

Description

Multicolor flow cytometric analysis of CX3CR1 expression on mouse splenocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with APC Rat Anti-Mouse CD11b Cat. No. 553312/561690 and either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse CX3CR1 antibody Cat. No. 567825; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CX3CR1 versus CD11b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CX3CR1 expression on mouse splenocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with APC Rat Anti-Mouse CD11b Cat. No. 553312/561690 and either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse CX3CR1 antibody Cat. No. 567825; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CX3CR1 versus CD11b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CX3CR1 expression on mouse splenocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with APC Rat Anti-Mouse CD11b Cat. No. 553312/561690 and either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse CX3CR1 antibody Cat. No. 567825; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CX3CR1 versus CD11b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CX3CR1 expression on mouse splenocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with APC Rat Anti-Mouse CD11b Cat. No. 553312/561690 and either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse CX3CR1 antibody Cat. No. 567825; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CX3CR1 versus CD11b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CX3CR1 expression on mouse splenocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with APC Rat Anti-Mouse CD11b Cat. No. 553312/561690 and either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse CX3CR1 antibody Cat. No. 567825; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CX3CR1 versus CD11b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CX3CR1 expression on mouse splenocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with APC Rat Anti-Mouse CD11b Cat. No. 553312/561690 and either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse CX3CR1 antibody Cat. No. 567825; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CX3CR1 versus CD11b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.