BD HorizonTM BV510 Rat Anti-Mouse F4/80

Description

Multicolor flow cytometric analysis of F4/80 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat Anti-CD11b antibody Cat. No. 562605 and with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse F4/80 antibody Cat. No. 569615; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of F4/80 expression on viable Mouse peritoneal cells. C57BL/6 Mouse peritoneal exudate cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567471 and with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse F4/80 antibody Cat. No. 569615; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD117 c-kit was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peritoneal cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of F4/80 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat Anti-CD11b antibody Cat. No. 562605 and with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse F4/80 antibody Cat. No. 569615; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of F4/80 expression on viable Mouse peritoneal cells. C57BL/6 Mouse peritoneal exudate cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567471 and with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse F4/80 antibody Cat. No. 569615; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD117 c-kit was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peritoneal cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of F4/80 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat Anti-CD11b antibody Cat. No. 562605 and with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse F4/80 antibody Cat. No. 569615; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of F4/80 expression on viable Mouse peritoneal cells. C57BL/6 Mouse peritoneal exudate cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567471 and with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952; Left Plot or BD Horizon BV510 Rat Anti-Mouse F4/80 antibody Cat. No. 569615; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD117 c-kit was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peritoneal cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.