BD HorizonTM BV510 Rat Anti-Pax-5

Description

Multicolor flow cytometric analysis of Pax5 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC and BALB/c mouse splenic leucocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon BV510 Rat Anti-Mouse Pax5 antibody Cat. No. 563191 or BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952 . The PBMC were counterstained with PE Mouse Anti-Human CD21 antibody Cat. No. 555422/557327/561768 . The mouse splenic leucocytes were counterstained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Panel A. Human Peripheral Blood Lymphocytes: The two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Left Plot and Pax5 Right Plot versus CD21 for events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes. Panel B. Mouse Splenocytes: The dot plots show the coexpression patterns of Ig Isotype control staining Left Plot or Pax5 Right Plot versus CD45R/B220 for events with the light-scatter characteristics of intact mouse splenocytes. Multicolor flow cytometric analysis of Pax5 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC and BALB/c mouse splenic leucocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon BV510 Rat Anti-Mouse Pax5 antibody Cat. No. 563191 or BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952 . The PBMC were counterstained with PE Mouse Anti-Human CD21 antibody Cat. No. 555422/557327/561768 . The mouse splenic leucocytes were counterstained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Panel A. Human Peripheral Blood Lymphocytes: The two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Left Plot and Pax5 Right Plot versus CD21 for events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes. Panel B. Mouse Splenocytes: The dot plots show the coexpression patterns of Ig Isotype control staining Left Plot or Pax5 Right Plot versus CD45R/B220 for events with the light-scatter characteristics of intact mouse splenocytes. Show More Multicolor flow cytometric analysis of Pax5 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC and BALB/c mouse splenic leucocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon BV510 Rat Anti-Mouse Pax5 antibody Cat. No. 563191 or BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952 . The PBMC were counterstained with PE Mouse Anti-Human CD21 antibody Cat. No. 555422/557327/561768 . The mouse splenic leucocytes were counterstained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Panel A. Human Peripheral Blood Lymphocytes: The two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Left Plot and Pax5 Right Plot versus CD21 for events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes. Panel B. Mouse Splenocytes: The dot plots show the coexpression patterns of Ig Isotype control staining Left Plot or Pax5 Right Plot versus CD45R/B220 for events with the light-scatter characteristics of intact mouse splenocytes.