BD HorizonTM BV605 Hamster Anti-Mouse CD279 (PD-1)

Description

Flow cytometric analysis of CD279 PD-1 on resting and activated mouse splenocytes. Mouse splenic leucocytes were either not activated or were activated by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 antibody Cat. No. 553057 for 3 days 37C . The resting Left Panel or activated Right Panel cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV605 Hamster IgG2, kappa Isotype Control Cat. No. 563012; dashed line histogram or BD Horizon BV605 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 563059; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD279 PD-1 on resting and activated mouse splenocytes. Mouse splenic leucocytes were either not activated or were activated by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 antibody Cat. No. 553057 for 3 days 37C . The resting Left Panel or activated Right Panel cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV605 Hamster IgG2, kappa Isotype Control Cat. No. 563012; dashed line histogram or BD Horizon BV605 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 563059; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD279 PD-1 on resting and activated mouse splenocytes. Mouse splenic leucocytes were either not activated or were activated by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 antibody Cat. No. 553057 for 3 days 37C . The resting Left Panel or activated Right Panel cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV605 Hamster IgG2, kappa Isotype Control Cat. No. 563012; dashed line histogram or BD Horizon BV605 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 563059; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD279 PD-1 on resting and activated mouse splenocytes. Mouse splenic leucocytes were either not activated or were activated by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 antibody Cat. No. 553057 for 3 days 37C . The resting Left Panel or activated Right Panel cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV605 Hamster IgG2, kappa Isotype Control Cat. No. 563012; dashed line histogram or BD Horizon BV605 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 563059; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD279 PD-1 on resting and activated mouse splenocytes. Mouse splenic leucocytes were either not activated or were activated by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 antibody Cat. No. 553057 for 3 days 37C . The resting Left Panel or activated Right Panel cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV605 Hamster IgG2, kappa Isotype Control Cat. No. 563012; dashed line histogram or BD Horizon BV605 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 563059; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD279 PD-1 on resting and activated mouse splenocytes. Mouse splenic leucocytes were either not activated or were activated by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 antibody Cat. No. 553057 for 3 days 37C . The resting Left Panel or activated Right Panel cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV605 Hamster IgG2, kappa Isotype Control Cat. No. 563012; dashed line histogram or BD Horizon BV605 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 563059; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.