BD HorizonTM BV605 Hamster Anti-Mouse CD49b (Integrin alpha2)

Description

Multicolor flow cytometric analysis of CD49b Integrin alpha2 expression on viable Mouse NK cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and with either BD Horizon BV605 Hamster IgG1, kappa Isotype Control Cat. No. 563009; Left Plot or BD Horizon BV605 Hamster Anti-Mouse CD49b Integrin alpha2 Cat. No. 569508; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD49b Integrin alpha2 or Ig Isotype control staining versus NK1.1 was derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD49b Integrin alpha2 expression on viable Mouse NK cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and with either BD Horizon BV605 Hamster IgG1, kappa Isotype Control Cat. No. 563009; Left Plot or BD Horizon BV605 Hamster Anti-Mouse CD49b Integrin alpha2 Cat. No. 569508; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD49b Integrin alpha2 or Ig Isotype control staining versus NK1.1 was derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD49b Integrin alpha2 expression on viable Mouse NK cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and with either BD Horizon BV605 Hamster IgG1, kappa Isotype Control Cat. No. 563009; Left Plot or BD Horizon BV605 Hamster Anti-Mouse CD49b Integrin alpha2 Cat. No. 569508; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD49b Integrin alpha2 or Ig Isotype control staining versus NK1.1 was derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD49b Integrin alpha2 expression on viable Mouse NK cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and with either BD Horizon BV605 Hamster IgG1, kappa Isotype Control Cat. No. 563009; Left Plot or BD Horizon BV605 Hamster Anti-Mouse CD49b Integrin alpha2 Cat. No. 569508; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD49b Integrin alpha2 or Ig Isotype control staining versus NK1.1 was derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD49b Integrin alpha2 expression on viable Mouse NK cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and with either BD Horizon BV605 Hamster IgG1, kappa Isotype Control Cat. No. 563009; Left Plot or BD Horizon BV605 Hamster Anti-Mouse CD49b Integrin alpha2 Cat. No. 569508; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD49b Integrin alpha2 or Ig Isotype control staining versus NK1.1 was derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD49b Integrin alpha2 expression on viable Mouse NK cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Mouse Anti-Mouse NK-1.1 antibody Cat. No. 550627 and with either BD Horizon BV605 Hamster IgG1, kappa Isotype Control Cat. No. 563009; Left Plot or BD Horizon BV605 Hamster Anti-Mouse CD49b Integrin alpha2 Cat. No. 569508; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD49b Integrin alpha2 or Ig Isotype control staining versus NK1.1 was derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.