BD HorizonTM BV605 Mouse Anti-Human CD103

Description

Flow cytometric analysis of CD103 expression on stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days and stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; dashed line histogram or BD Horizon BV605 Mouse Anti-Human CD103 antibody Cat. No. 569162; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expression of CD103 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD103 expression on stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days and stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; dashed line histogram or BD Horizon BV605 Mouse Anti-Human CD103 antibody Cat. No. 569162; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expression of CD103 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD103 expression on stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days and stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; dashed line histogram or BD Horizon BV605 Mouse Anti-Human CD103 antibody Cat. No. 569162; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expression of CD103 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.