
569303 · 25 Tests
BDB Bioscience · Cat: 569303
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD107a LAMP-1 expression in Jurkat cells. Cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; dashed line histogram or BD Horizon BV605 Mouse Anti-Human CD107a LAMP-1 antibody Cat. No. 569302/569303; solid line histogram . The fluorescence histogram showing CD107a LAMP-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD107a LAMP-1 expression in Jurkat cells. Cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; dashed line histogram or BD Horizon BV605 Mouse Anti-Human CD107a LAMP-1 antibody Cat. No. 569302/569303; solid line histogram . The fluorescence histogram showing CD107a LAMP-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD107a LAMP-1 expression in Jurkat cells. Cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; dashed line histogram or BD Horizon BV605 Mouse Anti-Human CD107a LAMP-1 antibody Cat. No. 569302/569303; solid line histogram . The fluorescence histogram showing CD107a LAMP-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.