BD HorizonTM BV605 Mouse Anti-Human CD16

Description

Multiparameter flow cytometric analysis of CD16 expression on Human peripheral blood leucocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD56 antibody Cat. No. 563554/563555; Top Plots and with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plots or BD Horizon BV605 Mouse Anti-Human CD16 antibody Cat. No. 569175; Right Plots at 1 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots: The bivariate pseudocolor density plot showing the correlated expression of CD16 or Ig Isotype control staining versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: The bivariate pseudocolor density plot showing CD16 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD16 expression on Human peripheral blood leucocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD56 antibody Cat. No. 563554/563555; Top Plots and with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plots or BD Horizon BV605 Mouse Anti-Human CD16 antibody Cat. No. 569175; Right Plots at 1 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots: The bivariate pseudocolor density plot showing the correlated expression of CD16 or Ig Isotype control staining versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: The bivariate pseudocolor density plot showing CD16 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD16 expression on Human peripheral blood leucocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD56 antibody Cat. No. 563554/563555; Top Plots and with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plots or BD Horizon BV605 Mouse Anti-Human CD16 antibody Cat. No. 569175; Right Plots at 1 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots: The bivariate pseudocolor density plot showing the correlated expression of CD16 or Ig Isotype control staining versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: The bivariate pseudocolor density plot showing CD16 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.