
569594 ·
BDB Bioscience · Cat: 569594
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multicolor flow cytometric analysis of CD34 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708 and with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD34 antibody Cat. No. 569594; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable CD14-negative DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD34 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708 and with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD34 antibody Cat. No. 569594; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable CD14-negative DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD34 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708 and with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD34 antibody Cat. No. 569594; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable CD14-negative DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD34 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708 and with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD34 antibody Cat. No. 569594; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable CD14-negative DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD34 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708 and with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD34 antibody Cat. No. 569594; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable CD14-negative DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD34 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708 and with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD34 antibody Cat. No. 569594; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable CD14-negative DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.